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Präzise und Phenol Freie DNA Geschlechtsbestimmung von Tag 30 Schweineembryonen mittels PCR
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
DOI:

10:16 min

February 14, 2016

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Chapters

  • 00:05Title
  • 01:12Grinding Embryos
  • 02:35Genomic DNA Preparation Using a Modified Sodium Hydroxide Method
  • 04:40Design Sex-specific PCR Primers and Genomic DNA PCR
  • 07:34Results: Porcine Embryo Sex Determination
  • 08:54Conclusion

Summary

Automatic Translation

This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

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