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Neuroscience
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激光制导神经跟踪在脑外植体
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Laser-guided Neuronal Tracing In Brain Explants
Please note that all translations are automatically generated.
Click here for the English version.
激光制导神经跟踪在脑外植体
DOI:
10.3791/53333-v
•
06:40 min
•
November 25, 2015
•
Otto Albrecht
,
Achim Klug
1
Department of Physiology and Biophysics
,
University of Colorado School of Medicine
Chapters
00:05
Title
00:56
Optical Genotyping
02:25
Preparing for the Tracer Injections
03:05
Anterograde/Retrograde Tracing
05:01
Results: Labeling Connections within the Trapezoid Body
05:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种技术,通过顺行或逆行示踪剂注射标记的神经元和它们的过程变成
使用
体外制备脑核。我们修改的
I
N通过利用荧光标记的小鼠突变体和基本的光学设备,以便增加贴标精度
体外
示踪剂电穿孔的现有方法。
Tags
Laser-guided Neuronal Tracing
Brain Explants
In Vitro Tracer Injection
Electroporation
Fluorescent Genetic Markers
Band-pass Filter Goggles
Neurocircuit Functionality
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