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Chapters
- 00:00Title
- 01:24Delipidation
- 03:52Depolymerization: Methanolysis with Sodium Methoxide
- 07:07Preparation of Trimethylsilyl Derivatives
- 07:54GC-MS Analysis
- 08:09Representative Results
- 09:01Conclusions
Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.
