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JoVE Journal
Immunology and Infection

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Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
 

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Article DOI: 10.3791/53389-v 11:11 min February 8th, 2016
February 8th, 2016

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Summary

Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.

Tags

Keywords: Sertoli Cells Peritubular Cells Rat Testes Cell Isolation Testicular Macrophages Autophagy Male Infertility Seminiferous Tubules Cell Culture Primary Cells
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