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JoVE Journal Chemistry
Water in Oil Emulsions
 

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Article DOI: 10.3791/53410-v • 11:26 min • March 21st, 2016
March 21st, 2016 •
1, 2
1Department of Biological Chemistry, Weizmann Institute of Science, 2Migal-Galilee Research Institute

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Summary
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This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.

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Keywords: Water In Oil Emulsions Chlorophyll-binding Proteins Hydrophobic Pigments Chlorophyll Extraction Spirulina Platensis Cyanobacteria DEAE-Sepharose Acetone Methanol Diethyl Ether Protein-pigment Complexes
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