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Journal
/
Bioengineering
/
中空纤维生物反应器的<em>在体内</em>般的哺乳动物组织培养
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Hollow Fiber Bioreactors for
In Vivo
-like Mammalian Tissue Culture
Please note that all translations are automatically generated.
Click here for the English version.
中空纤维生物反应器的<em>在体内</em>般的哺乳动物组织培养
DOI:
10.3791/53431-v
•
08:28 min
•
May 26, 2016
•
Michael P. Storm
,
Ian Sorrell
,
Rebecca Shipley
,
Sophie Regan
,
Kim A. Luetchford
,
Jean Sathish
,
Steven Webb
,
Marianne J. Ellis
1
Department of Chemical Engineering and Centre for Regenerative Medicine
,
University of Bath
,
2
MRC Centre for Drug Safety Science and Institute of Translational Medicine
,
University of Liverpool
,
3
Mechanical Engineering
,
University College London
,
4
Department of Applied Mathematics
,
Liverpool John Moores University
Chapters
00:05
Title
00:35
Module Fabrication
01:58
System Setup and Sterilization
04:12
Seeding the Module
06:01
Excision of Fibers
06:44
Results: Seeding, Proliferation, Viability and Biochemical Analysis
08:06
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
在培养细胞的功能行为可以通过在更
体内
样的三维培养环境
16-21
培养得到改善。该原稿描述了一种中空纤维生物反应器系统的
用于体内
式的哺乳动物组织培养的设置和操作。
Tags
Keywords: Hollow Fiber Bioreactor
In Vitro Model
Liver Cell Function
Physiologically Relevant Environment
Sinusoid
Bile Canaliculi
Cell Culture
Drug Dosing
Borosilicate Glass
Siliconizing
Autoclave
Silicone Glue
PTFE Tape
Ethanol Sterilization
Pump Tubing
Module Connectors
Permeate Line
Retentate Line
Article
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