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Saflaştırma ve Amiloid fibrillerin kadar yeniden katlama (His)<sub> 6</sub> etiketli Rekombinant Shadoo Protein Eklenmesi Organları olarak ifade<em> E.coli</em
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JoVE Journal Biology
Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli
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Saflaştırma ve Amiloid fibrillerin kadar yeniden katlama (His)<sub> 6</sub> etiketli Rekombinant Shadoo Protein Eklenmesi Organları olarak ifade<em> E.coli</em

DOI:
10.3791/53432-v

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09:43 min

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December 19, 2015

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, , ,
1Virologie et Immunologie Moléculaires,INRA

Chapters

  • 00:05Title
  • 00:43Shadoo Protein Expression and Purification by Low Pressure Liquid Chromatography
  • 03:07Ni2+-affinity Chromatography
  • 04:51Size Exclusion Chromatography and Desalting
  • 06:07In Vitro Assembly of Shadoo into Amyloid Fibrils at Physiological pH and Acidic pHs
  • 08:01Results: His6-tagged Shadoo Protein Purification and Refolding to Amyloid Fibrils
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

A two-step chromatographic method is described for the purification of recombinant Shadoo protein expressed as inclusion bodies in Escherichia coli, as well as a protocol to fibrillate purified Shadoo into amyloid structures.

Tags

Keywords: Recombinant Shadoo ProteinInclusion BodiesPurificationAffinity ChromatographySize Exclusion ChromatographyAmyloid FibrilsPrion Diseases

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