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Immunology and Infection

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侵染微生物利用数字条码平台的基因表达谱

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Please note that all translations are automatically generated. Click here for the English version.

侵染微生物利用数字条码平台的基因表达谱

DOI:

10.3791/53460-v

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09:13 min

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January 13, 2016

•

Wenjie Xu, Norma V. Solis, Scott G. Filler, Aaron P. Mitchell

¹Department of Biological Sciences,Carnegie Mellon University, ²Infectious Diseases,Harbor-UCLA Medical Center

Chapters

00:05Title
00:59Preparation of Reagents and Instruments
01:47RNA Extraction from Infected Tissues
04:01Gene Expression Profiling Using Digital Bar-coding
06:55Results: C. albicans Gene Expression Using Digital Bar-coding
08:21Conclusion

Summary

Automatic Translation

We developed a protocol that is fast, sensitive and reproducible for pathogen gene expression profiling during an infection.

Tags

Keywords: Gene Expression Profiling Digital Bar-coding Pathogen Gene Expression Infectious Disease C. Albicans RNA Extraction Phenol-chloroform Extraction

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