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Optical Control of Living Cells Electrical Activity by Conjugated Polymers
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Bioengineering
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JoVE Journal Bioengineering
Optical Control of Living Cells Electrical Activity by Conjugated Polymers

Optical Control of Living Cells Electrical Activity by Conjugated Polymers

DOI:
10.3791/53494-v

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10:16 min

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January 28, 2016

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, , , ,
1Center for Nano Science and Technology @PoliMi,Istituto Italiano di Tecnologia, 2Dipartimento di Fisica,Politecnico di Milano

Chapters

  • 00:05Title
  • 01:18Preparation of Photoactive Substrates
  • 02:36Plating HEK-293 Cells on the Active Substrates
  • 04:21Optical Stimulation Protocol and Electrophysiological Measurements
  • 07:48Results: Optical Stimulation of HEK-293 Cells
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

A method for stimulation of in-vitro cell cultures electrical activity with visible light, based on the use of organic semiconducting polymers is described.

Tags

Keywords: Optical ControlLiving CellsElectrical ActivityConjugated PolymersPhototransductionOptogeneticsThermal StimulationP3HTPCBMIndium Tin OxideSpincoatingSterilizationPolydimethylsiloxane

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