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药物与DNA相互作用的细胞全基因组映射与COSMIC（小分子交联，染色质隔离）

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Biology

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JoVE Journal Biology

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

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药物与DNA相互作用的细胞全基因组映射与COSMIC（小分子交联，染色质隔离）

DOI:

10.3791/53510-v

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10:05 min

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January 20, 2016

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Graham S. Erwin, Matthew P. Grieshop, Devesh Bhimsaria², Asuka Eguchi, José A. Rodríguez-Martínez, Aseem Z. Ansari⁴

¹Department of Biochemistry,University of Wisconsin–Madison, ²Department of Electrical and Computer Engineering,University of Wisconsin–Madison, ³Graduate Program in Cellular and Molecular Biology,University of Wisconsin–Madison, ⁴The Genome Center,University of Wisconsin–Madison

Chapters

00:05Title
00:48Crosslinking in Live Cells
03:19Isolation of Chromatin
05:14Capture of Ligand-DNA Crosslinks and Isolation of Affinity-purified DNA
07:52Results: COSMIC Indentifies Genome-wide Binding Events of Polyamides
09:21Conclusion

Summary

Automatic Translation

识别基因组靶向分子的直接目标仍然是一个重大的挑战。要了解DNA结合分子如何参与基因组，我们开发了依靠小分子交联，染色质隔离的方法（COSMIC）。

Tags

Keywords: Genome-wide Mapping Drug-DNA Interactions COSMIC Crosslinking Small Molecules Chromatin Polyamides DNA-targeting Mechanism Of Action Therapeutic Agents DNA-targeting Molecules Pluripotent Stem Cells Photo-cross-linking UV Radiation Cell Dissociation Enzyme E8 Media

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