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的策略,以验证胼胝质介导的Plasmodesmal浇注在热带反应中的作用
JoVE Journal
Biology
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JoVE Journal Biology
A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
DOI:

12:18 min

April 17, 2016

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Chapters

  • 00:05Title
  • 01:12Screening for Altered Phototropic or Gravitropic Responses
  • 04:09Analysis of Seedling Bending Angles
  • 05:32Hypocotyl Assay: HPTS Dye-loading
  • 07:30Callose Aniline Blue Staining
  • 08:44Analysis of Callose Signal
  • 09:45Results: Screening AtGSL8 RNAi Plants
  • 11:06Conclusion

Summary

Automatic Translation

本文介绍了筛选基因控制的热带响应期间plasmodesmal渗透性,因此生长素梯度的方法。这包括在胚轴热带反应程度的测量 拟南芥和8羟基芘-1,3,6-三磺酸(HPTS)装载终于胼胝质水平评估检查plasmodesmal渗透性。

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