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Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

DOI:
10.3791/53585-v

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11:28 min

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March 25, 2016

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, ,
1David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, Rochester, NY

Chapters

  • 00:05Title
  • 00:56Effector T Cell Injection
  • 02:28Mouse Imaging Preparation
  • 05:21In vivo Time-lapse Imaging and Intravenous Antibody Administration
  • 08:51Results: Representative In Situ Adoptively Trabsferred Effector T Cell Imaging
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

The mechanisms that govern the interstitial motility of CD4 effector T cells at sites of inflammation are relatively unknown. We present a non-invasive approach to visualize and manipulate in vitro-primed CD4 T cells in the inflamed ear dermis, allowing for study of the dynamic behavior of these cells in situ.

Tags

Keywords: CD4 T CellsInterstitial MigrationInflamed DermisIn Situ ImagingEffector T CellsFluorescent LabelingTail Vein InjectionFluorescent EmulsionEar DermisAnesthetizationTail Vein CatheterizationImmunologyImmune Cell Dynamics

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