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1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences,KU Leuven, 2Division of Nuclear Medicine,Leuven University Hospital – KU Leuven, 3Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences,KU Leuven, 4Leuven Viral Vector Core,KU Leuven
Chapters
- 00:05Title
- 01:11Stereotactic Injection of rAAV-α-SYN into the Rat Substantia Nigra
- 05:18Results: Neurodegeneration Induced by rAAV2/7 α-SYN
- 07:30Conclusion
This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.
Tags
Parkinson’s DiseaseAlpha-synucleinRat ModelStereotactic InjectionRecombinant Adeno-associated Viral VectorViral Vector-based Animal ModelPre-clinical Drug TestingNeurodegenerative DisordersBrain Region TargetingHigh Transgene ExpressionVector System ChoiceAnesthesiaMicroTransponderAseptic TechniqueStereotaxic FrameScalp DisinfectionMicroinjection SyringeRecombinant AAV
