Journal
/
/
Identifikation af kritiske forhold for Immunfarvning i Ærten Bladlus embryoner: Stigende Tissue permeabilitet og aftagende baggrundsfarvning
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining
DOI:

09:44 min

February 02, 2016

,

Chapters

  • 00:05Title
  • 00:58Culture of Aphids
  • 02:06Dissection and Fixation of Ovaries
  • 03:02Treatment of Ovaries
  • 04:09Antibody Staining
  • 05:09Mounting the Aphid Embryos
  • 07:00Results: Aphid Ovary Dissection and Whole-mount Immunostaining with Aphid-specific Conditions to Increase Tissue Permeability and Decrease Background Staining
  • 08:11Conclusion

Summary

Automatic Translation

A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.

Related Videos

Read Article