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Chapters
- 00:05Title
- 00:45Whole-cell Biocatalysts Preparation
- 02:05Biosynthesis of L-xylulose by Coupling E. coliHjLAD and E. coliSpNOx for Cofactor Regeneration
- 03:51Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads
- 06:25Stability Assay of Immobilized Biocatalysts for L-xylulose Production
- 07:15Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability
- 08:55Conclusion
Præsenteret er protokollen for co-immobilisering hel-celle biokatalysatorer til cofaktor regenerering og forbedret genanvendelighed, ved hjælp af produktion af L-xylulose som eksempel. Cofaktoren regenerering opnås ved kobling to Escherichia coli stammer, der udtrykker funktionelt komplementære enzymer; helcelle-biokatalysator immobilisering opnås ved celleindkapsling i calciumalginatperler.
