A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:01Biological Sample Permethylation
- 03:58Liquid/Liquid Extraction and Glycan Purification
- 05:24Trifluoroacetic Acid (TFA) Hydrolysis of Glycans
- 06:19Glycan Reduction
- 07:13Glycan Acetylation
- 08:36Gas Chromatography – Mass Spectrometry (GC-MS)
- 09:35Results: Optimized Route for Permethylation
- 11:03Conclusion
This article presents an enhanced form of a novel bottom-up glycomics technique designed to analyze the pooled compositional profile of glycans in unfractionated biofluids through the chemical breakdown of glycans into their constituent linkage-specific monosaccharides for detection by GC-MS. Potential applications include early detection of cancer and other glycan-affective disorders.
