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Journal / Developmental Biology / Using Light Sheet Fluorescence Microscopy to…
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Developmental Biology

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JoVE Journal Developmental Biology
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
 

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Article DOI: 10.3791/53966-v • 13:01 min • April 10th, 2016
April 10th, 2016 •
*1, *1, 1, 1, 1,2,3, 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center
* These authors contributed equally

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Summary
Automatic Translation

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Keywords: Light Sheet Fluorescence Microscopy Zebrafish Eye Development Multiview Reconstruction Low Phototoxicity Fast Acquisition 3D Volume Developmental Biology Neuroscience Agarose E3 Medium MS 222 Fluorescent Beads
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