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Eine ganze Berg<em> In Situ</em> Hybridisierungsverfahren für die Gastropod Mollusc<em> Spitzschlammschnecke</em
JoVE Journal
Biology
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JoVE Journal Biology
A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
DOI:

07:33 min

March 15, 2016

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Chapters

  • 00:05Title
  • 00:52Assembly of Decapsulation Apparatus
  • 01:43Sample Collection, Fixation and Decapsulation
  • 05:48Results: Representative Images of WMISH Expression Patterns Against a Variety of Genes from a Range of L. stagnalis Developmental Stages
  • 06:42Conclusion

Summary

Automatic Translation

The goal of this protocol is to provide users with a set of methods for the high-throughput decapsulation of Lymnaea stagnalis embryos and larvae in preparation for whole mount in situ hybridization, and for subsequent pre- and post-hybridization treatments.

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