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In vivo Biosensor Tracks Ikke-apoptotisk Caspase aktivitet i Drosophila
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In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In vivo Biosensor Tracks Ikke-apoptotisk Caspase aktivitet i Drosophila

DOI:
10.3791/53992-v

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13:21 min

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November 27, 2016

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, , ,
1W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 2School of Life Sciences,Chinese University of Hong Kong

Chapters

  • 00:05Title
  • 01:48Preparing the CaspaseTracker Flies and Dissected Tissues
  • 04:30Tissue Staining and Mounting
  • 08:11Confocal Microscopy
  • 09:37Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

For at detektere sunde celler i hele dyr, der indeholder lave niveauer af caspaseaktivitet, blev den meget følsomme biosensor betegnet CaspaseTracker genereret for Drosophila. Caspase-afhængige biosensor aktivitet detekteres i langlivede raske celler i hele de indre organer af voksne dyr, der er opdrættet under optimerede betingelser i fravær af død stimuli.

Tags

Caspase ActivityIn Vivo BiosensorDrosophila MelanogasterNon-apoptotic CaspaseCaspase TrackerG-TraceDQVDGal4RFPFLP RecombinaseGFPCaspase Cleavage Site

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