/

/

用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Please note that all translations are automatically generated. Click here for the English version.

用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成

DOI:

10.3791/54019-v

•

12:31 min

•

May 05, 2016

•

Sharareh Shojaie, Joyce Lee, Jinxia Wang, Cameron Ackerley, Martin Post

¹Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning,Hospital for Sick Children

Chapters

00:05Title
00:47Scaffold Preparation
03:57Thick Section Generation
07:17Decontamination of Scaffold Sections and Recellularization Setup
09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
11:52Conclusion

Summary

Automatic Translation

该协议有效地引导小鼠胚胎干细胞衍生定形内胚层成熟呼吸道上皮细胞。这种分化技术使用的3维脱细胞肺支架直接肺谱系说明书中，在一个确定的，无血清培养的设置。

Tags

Keywords: Decellularized Lung Scaffolds Embryonic Stem Cell Differentiation Airway Epithelial Cells 3D Culture Lung Development Cell Matrix Contractions Growth Factor Signaling Serum-free Setting Robust Airway Epithelial Differentiation Limited Endoderm Lineage Contamination Rat Lungs Perfusion Tracheal Cannulation Decellularization Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

上皮细胞复育和肾组织的发展准备啮齿动物细胞外基质的支架

脱碳软骨衍生基质支架的制备

由去细胞化肺切片制备的工程肺组织

用于体外和体内骨组织工程的脱细胞苹果衍生支架

区域特异性去细胞化肺组织的解剖和分离

Development and Characterization of Decellularized Lung Extracellular Matrix Hydrogels

人上皮肺癌细胞培养和细胞表面增强拉曼散射（SERS）实验

脱细胞苹果衍生支架的制备和细胞接种

共聚焦激光扫描显微镜对脱细胞苹果衍生支架的孔径测量和细胞分布分析

脱细胞苹果衍生支架中的碱性磷酸酶活性、钙沉积和 Young 模量测量

Read Article