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Det<em> Ex vivo</em> Kultur og Pattern Recognition Receptor Stimulering af Mouse Intestinal Organoids
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Immunology and Infection
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JoVE Journal Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Det<em> Ex vivo</em> Kultur og Pattern Recognition Receptor Stimulering af Mouse Intestinal Organoids

DOI:
10.3791/54033-v

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09:09 min

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May 18, 2016

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, , , ,
1Department of Biomedical Sciences and Pathobiology,Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering,Cornell University, 3School of Electrical and Computer Engineering,Cornell University, 4Department of Biomedical Engineering,Duke University

Chapters

  • 00:05Title
  • 00:46Mus musculus Small Intestinal Crypt Harvest
  • 03:57Organoid Plating for Pathogen Associated Molecular Patters (PAMP) and L. monocytogenes Challenge
  • 06:58Results: Representative Mouse Intestinal Organoid Culture and Stimulation
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Her er en protokol til at høste, vedligeholde og behandle mus små tarm organoids med patogen forbundet molekylære mønstre (PAMPs) og Listeria monocytogenes beskrevet, samt fokus på genekspression og ordentlig normalisering teknikker til protein.

Tags

Mouse Intestinal OrganoidsEx Vivo CulturePattern Recognition Receptor StimulationInnate ImmunityMucosal ImmunologyHost-pathogen InteractionsIntestinal Epithelial CellsELISAIntestinal CryptsNormalizationCell NumberPBSEDTADMEM/F12Cell FractionationCrypt Visualization

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