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1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry,Western University, 3Department of Biochemistry, Schulich School of Medicine & Dentistry,Western University, 4Department of Oncology, Schulich School of Medicine & Dentistry,Western University, 5Lawson Health Research Institute
Chapters
- 00:05Title
- 00:47Lymph Node (LN), Lung, Leg Bones, and Brain Isolation
- 03:29Lung- or Brain-conditioned Medium Generation
- 04:53Bone Marrow- or LN-conditioned Medium Generation
- 06:58Results: Representative Organ-specific Influences on Breast Cancer Metastatic Behavior
- 08:35Conclusion
This manuscript describes an ex vivo model system comprised of organ-conditioned media derived from the lymph node, bone, lung, and brain of mice. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
