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Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

DOI:
10.3791/54070-v

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09:34 min

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June 22, 2016

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, ,
1Department of Medical Microbiology and Immunology,University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine,Yale University School of Medicine, 3Department of Pathophysiology,Southern Medical University (China)

Chapters

  • 00:05Title
  • 00:47Phenotyping pIL1-DsRed Mice
  • 02:51Skin Inflammation Test
  • 04:17Labeling Neutrophils
  • 05:39Intravital Imaging of Neutrophil Priming in pIL-1-DsRed Mice
  • 07:11Results: Neutrophil Priming Dynamics
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

Tags

Keywords: Intravital ImagingNeutrophil PrimingIL-1β PromoterDsRed Reporter MiceFluorescent LabelingFlow CytometryAnesthesiaEar Thickness MeasurementOxazolone

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