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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
 

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Article DOI: 10.3791/54090-v • 11:52 min • August 4th, 2016
August 4th, 2016 •
1, 1, 1, 1,2
1Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center, The Ohio State University, 2Department of Pharmacology, The Ohio State University

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Summary
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We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.

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Keywords: Targeted RNA Sequencing Gene Expression Genomic Alterations Ribosomal RNA Depletion Chemical Fragmentation CDNA Synthesis Barcoded Libraries Hybridization Target Capture Multiplexed Sequencing Gene Fusions Single Nucleotide Variations Alternative Splicing Custom Probes Cot-1 DNA Blocking Oligos Hybridization Buffer Streptavidin Beads
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