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A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

DOI:
10.3791/54104-v

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09:27 min

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June 04, 2016

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, , ,
1Faculty of Medicine and Pharmacy, Research Unit for Infectious and Tropical Emerging Diseases,Aix Marseille University, 2Pole of Infectious and Tropical Diseases, Clinical and Biological Sector, Federation of Bacteriology-Hygiene Virology,University Hospital Institute Mediterranean Infection

Chapters

  • 00:05Title
  • 00:52Host Preparation for Blind Culture Procedures and Sample Inoculation
  • 02:59Detection of Lysis by Automated Flow Cytometry
  • 04:51Preliminary Staining to Reveal the Presence of Giant Viruses and Electron Microscopy
  • 06:59Results: Detection of Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

Tags

Keywords: FaustovirusesVermamoeba VermiformisVirus IsolationEnvironmental SamplesAntibiotic And Antifungal MixtureStarvation MediumFlow CytometryAmoeba CultureVirus ProspectingPan-genomeVirology

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