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Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
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Biochemistry
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JoVE Journal Biochemistry
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

DOI:
10.3791/54111-v

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09:04 min

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October 21, 2016

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, , ,
1School of Applied Science,Temasek Polytechnic, 2University of Applied Sciences Utrecht

Chapters

  • 00:05Title
  • 00:39Grafting Amine Functional Groups on Cellulose Paper Discs
  • 03:10Paper-based ELISA for IgG Detection
  • 06:28Results: Analysis of the Binding Activity of Immobilized Antibodies to the Covalent Immobilized Cellulose Paper Matrix
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.

Tags

Keywords: Covalent BindingAntibodyCellulose PaperColorimetric ImmunoassayPoint-of-care DiagnosticsPaper-based AssayAPSAmine GroupsFTIRGlutaraldehyde

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