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Journal / Biology / FRET Imaging in Three-dimensional Hydrogels
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FRET Imaging in Three-dimensional Hydrogels
 

FRET Imaging in Three-dimensional Hydrogels

Article DOI: 10.3791/54135-v • 09:47 min • August 1st, 2016
August 1st, 2016 •
*1, *1,3, 1,2
1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 3Laerdal AS
* These authors contributed equally

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Summary
Automatic Translation

Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.

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Keywords: FRET Imaging 3D Hydrogels Intracellular Signaling Developmental Biology Pharmacology Regenerative Medicine Biomaterials Drug Screening 3D Microtissues Wide Field Microscopy Hydrogel Casting Cell Suspension Crosslinking UV Exposure PDMS Dish Phenol-free Medium Microscope Imaging FRET Ratio
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