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Retrovirale Transduktion von Knochenmark-Progenitorzellen zu generieren T-Zell-Rezeptor Retrogenic Mäuse
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Chapters
- 00:05Title
- 01:01Day 0: Bone Marrow Cell Isolation and Culture of Retroviral Producer Cells
- 03:52Day 1: Retroviral Supernatant Collection and Harvest and Transduction of Bone Marrow Cells
- 05:37Day 2: Transduction of Bone Marrow Cells
- 06:39Day 4: Harvest of Transduced Bone Marrow
- 07:48Results: Example of Bones Marrow Transduction and Peripheral TCR Retrogenic T Cells
- 08:44Conclusion
We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.
