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Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
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Chapters
- 00:05Title
- 01:19Human Bone Marrow Mononuclear Cell (hBM-MNC) Isolation
- 02:52Mesangiogenic Progenitor Cell (MPC) Isolation from hBM-MNCs
- 04:01MPC Mesengenic Differentiation
- 05:39MPC Spheroid Sprouting Assay
- 06:47Results: Representative MNC Differentiation and Analyses
- 08:41Conclusion
Here we describe an optimized, highly reproducible protocol to isolate Mesodermal Progenitor Cells (MPCs) from human bone marrow (hBM). MPCs were characterized by flow cytometry and nestin expression. They showed the ability to give rise to exponentially growing MSC-like cell cultures while retaining their angiogenic potential.
