/
/
Isolamento de perivascular Multipotentes populações de células precursoras a partir de tecido cardíaco humano
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles
Chapters
- 00:05Title
- 01:06Tissue Digestion and Cell Isolation
- 03:27Cell Labelling and Sorting
- 06:05Cell Culture
- 06:54Results: FAS Purification of Myocardial Perivascular Cells and Perivascular Cell Morphology
- 07:39Conclusion
tecido cardíaco humano abriga populações de células precursoras perivasculares multipotentes que podem ser adequados para a regeneração do miocárdio. A técnica aqui descrita permite o isolamento e purificação simultânea de duas populações de células estromais multipotentes associados com os vasos nativos de sangue, ie CD146 + CD34 - pericitos e células CD34 + CD146 - células adventícias, a partir do miocárdio humano.
