Genetische en biochemische benaderingen voor<em> In Vivo</em> en<em> In Vitro</em> Evaluatie van Protein Oligomerisatie: De ryanodinereceptor Case Study
Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.