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Correlativo luce e microscopia elettronica Utilizzando Quantum Dot nanoparticelle
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1South Western Sydney Clinical School, Faculty of Medicine,University of New South Wales Australia, 2School of Medicine,Western Sydney University, 3Correlative Microscopy Group,Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service,New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine,University of New South Wales Australia
Chapters
- 00:05Title
- 00:53Immunolabeling
- 04:33Fluorescence Light Microscopy
- 06:12Transmission Electron Microscopy
- 07:10CLEM Imaging
- 08:44Results: Correlative Light and Electron Microscopic Imaging of Quantum Dot-labeled Tumor Cells
- 09:56Conclusion
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
