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Immunology and Infection

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JoVE Journal Immunology and Infection
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
 

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Article DOI: 10.3791/54312-v • 05:38 min • January 30th, 2017
January 30th, 2017 •
1, 1, 1, 1, 1, 1, 1
1Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Summary
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We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.

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Keywords: Influenza Viruses Rapid Molecular Detection Respiratory Infections Nasal Swab Viral Transport Media Sample Receiver Test Base Instrument Patient ID Sample Processing Transfer Cartridge
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