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微流控缓冲液更换为无干扰微/纳米颗粒细胞工程
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Bioengineering
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JoVE Journal Bioengineering
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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微流控缓冲液更换为无干扰微/纳米颗粒细胞工程

DOI:
10.3791/54327-v

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10:27 min

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July 10, 2016

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, , , ,
1Lee Kong Chian School of Medicine,Nanyang Technological University, 2School of Chemical and Biomedical Engineering,Nanyang Technological University, 3NTU-Northwestern Institute of Nanomedicine,Nanyang Technological University

Chapters

  • 00:05Title
  • 01:16Nanoparticle Labeling of Mesenchymal Stem Cells and Monocytes
  • 03:29Microfluidic Device Fabrication
  • 06:17Operating the Microfluidic Device
  • 09:04Results: Dean Flow Fractionation of Nano-particle Labeled Monocytes (THP-1)
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

这个协议描述了使用惯性基于微流体缓冲交换策略的纯化微/纳米工程细胞与未结合的颗粒的有效消耗。

Tags

Keywords: MicrofluidicBuffer ExchangeNanoparticleCell EngineeringInertial FocusingSpiral MicrochannelMesenchymal Stem CellsTHP-1 CellsSilica NanoparticlesPLGA Calcium NanoparticlesPoly-l-lysineCell LabelingCell PurificationHigh ThroughputRegenerative Medicine

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