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Microfluidic Buffer Exchange for Forstyrrelsesfri Micro / partikler Cell Engineering
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Bioengineering
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JoVE Journal Bioengineering
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Forstyrrelsesfri Micro / partikler Cell Engineering

DOI:
10.3791/54327-v

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10:27 min

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July 10, 2016

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, , , ,
1Lee Kong Chian School of Medicine,Nanyang Technological University, 2School of Chemical and Biomedical Engineering,Nanyang Technological University, 3NTU-Northwestern Institute of Nanomedicine,Nanyang Technological University

Chapters

  • 00:05Title
  • 01:16Nanoparticle Labeling of Mesenchymal Stem Cells and Monocytes
  • 03:29Microfluidic Device Fabrication
  • 06:17Operating the Microfluidic Device
  • 09:04Results: Dean Flow Fractionation of Nano-particle Labeled Monocytes (THP-1)
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver bruken av en treghets MicroFluidics basert bufferbytte strategi for å rense mikro / nanopartikkel modifiserte cellene med effektiv uttømming av ubundne partikler.

Tags

MicrofluidicBuffer ExchangeNanoparticleCell EngineeringInertial FocusingSpiral MicrochannelMesenchymal Stem CellsTHP-1 CellsSilica NanoparticlesPLGA Calcium NanoparticlesPoly-l-lysineCell LabelingCell PurificationHigh ThroughputRegenerative Medicine

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