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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
August 26th, 2016
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Sylvie A. Quideau1, Anne C.S. McIntosh2, Charlotte E. Norris1, Emily Lloret3, Mathew J.B. Swallow4, Kirsten Hannam5
1Department of Renewable Resources, University of Alberta, 2Department of Science, Augustana Faculty, University of Alberta, 3Laboratoire Génie Civil et géo-Environnement, Université de Lille, 4Department of Earth and Environmental Sciences, Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre, Natural Resources Canada
Chapters
Summary
Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.
Tags
Extraction Analysis Microbial Phospholipid Fatty Acids Soils Laboratory Protocol Soil Microbial Communities Land Management Changes Natural Disturbance Technique Easy-to-follow Robust Protocol Katelyn Pretzlaff Technician Research School Potassium Hydroxide Citrate Buffer Nonadeconoate Surrogate Standard Chloroform Bligh And Dyer Extraction Method Methanol Centrifuge SupernatantRelated Videos
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