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可再生生物燃料及生化生产的非常规酵母基因工程

JoVE Journal
Genetics

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JoVE Journal Genetics

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Please note that all translations are automatically generated. Click here for the English version.

可再生生物燃料及生化生产的非常规酵母基因工程

DOI:

10.3791/54371-v

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10:10 min

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September 20, 2016

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Ai-Qun Yu², Nina Pratomo², Tee-Kheang Ng², Hua Ling², Han-Saem Cho², Susanna Su Jan Leong^2,3, Matthew Wook Chang²

¹Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, ²NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute,National University of Singapore, ³Food Science and Chemical Engineering,Singapore Institute of Technology

Chapters

00:05Title
01:18Construction of the Disruption Cassette for Deleting KU70
03:08Generation of the Y. lipolytica KU70 Deletion Strain
05:45Cre Recombinase-mediated Marker Rescue
07:59Results: Deletion of KU70 Enhances Gene Deletion Efficiency
09:27Conclusion

Summary

Automatic Translation

We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

Tags

Genetic Engineering Unconventional Yeast Renewable Biofuel Biochemical Production Yarrowia Lipolytica PO1G Strain Gene Deletion Homologous Recombination Transformation Protocol LoxP Sites LEU2 Expression Cassette KU70 Gene Disruption Cassette

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