Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Purification of Native Complexes for Structural…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
 

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Article DOI: 10.3791/54389-v • 10:36 min • July 27th, 2016
July 27th, 2016 •
1, 1,2
1Department of Biochemistry, Brandeis University, 2Winship Cancer Institute, Emory University School of Medicine

Chapters

Summary
Automatic Translation

The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.

Tags

Keywords: Native Macromolecular Complex Purification Tandem Affinity Tag Structural Study S. Cerevisiae Cell Lysis Liquid Nitrogen Coffee Grinder Centrifugation Buffer Conditions Protein Complex
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter