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Affiniteit Labeling Detectie van endogene receptoren van zebravis embryo's
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Developmental Biology
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JoVE Journal Developmental Biology
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
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Affiniteit Labeling Detectie van endogene receptoren van zebravis embryo's

DOI:
10.3791/54405-v

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08:39 min

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August 31, 2016

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, ,
1Cell and Developmental Biology, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Chapters

  • 00:05Title
  • 00:47Preparation of Embryo Protein Extract
  • 03:22Detection of Endogenous Receptor Protein
  • 05:45Results: AFLIP Detection of BG in Zebrafish Embryos
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

Tags

Affinity LabelingReceptor DetectionZebrafish EmbryosDevelopmental BiologyReceptor ExpressionBiochemical PropertiesAFLIPCell BiologyDeyolkingYolk RemovalLigand BindingProtein ExtractionBradford Assay

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