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Immunology and Infection
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Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

DOI:
10.3791/54406-v

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09:25 min

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August 19, 2016

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1Department of Biological Sciences, Eck Institute for Global Health,University of Notre Dame

Chapters

  • 00:05Title
  • 00:58Bacterial Culture
  • 01:48Host Cell Infection with Permeable Membrane Insert System
  • 03:52Sample Collection
  • 06:13Results: Effect of Secreted Group A Streptococcus Toxins on Host Cells Using a Permeable Membrane Insert-based Infection System
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a method using a permeable membrane insert-based infection system to study the effects of Streptolysin S, a secreted toxin produced by Group A Streptococcus, on keratinocytes is described. This system can be readily applied to the study of other secreted bacterial proteins on various host cell types during infection.

Tags

Keywords: Permeable Membrane InsertHost-pathogen InteractionsSecreted Bacterial ToxinsGAS InfectionHaCaT CellsCell SignalingMembrane PermeabilizationLDH Release

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