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Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System
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Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

DOI:
10.3791/59517-v

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09:04 min

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May 28, 2019

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, ,
1Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 2Department of Biology,University of North Carolina at Chapel Hill

Chapters

  • 00:04Title
  • 00:24Preparation of Arabidopsis thaliana Seedlings on a Plastic Mesh
  • 01:36Colonization of Plants in Liquid Bacterial Growth Medium
  • 03:16Bacterial Colonization Maintenance
  • 04:14Collection of Bacteria for Viable Cell Counts
  • 05:19Collection of Intact Plant Roots for Microscopy
  • 06:13Results: Assessment of Bacterial Colonization on Arabidopsis thaliana Roots
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a hydroponic plant growth assay to quantify species presence and visualize the spatial distribution of bacteria during initial colonization of plant roots and after their transfer into different growth environments.

Tags

Bacterial ColonizationArabidopsis ThalianaRootsFloating Hydroponic SystemPlant-microbe InteractionsMesh DisksSeed GerminationBacterial Growth MediumColony-forming UnitsGas-permeable FilmPlant Growth Chamber

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