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Padronização Subtractive rápida de camadas de células vivas com uma Probe microfluídicos
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Chapters
- 00:05Title
- 01:16Microfluidic Probe (MFP) Head and Platform Cleaning and Preparation
- 06:13Creating the Hierarchical Hydrodynamic Flow Confinement (hHFC) and Patterning Cell Monolayers Using hHFC
- 08:10Downstream Processing for Sampling and DNA Amplification
- 09:46Results: Rapid Subtractive Patterning of Cell Monolayers Using a Hierachical HFC
- 10:54Conclusion
We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.
