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Bewertung der Wirksamkeit und Toxizität von RNAs Targeting HIV-1-Produktion für den Einsatz in Gene oder Arzneimitteltherapie
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
DOI:

12:03 min

September 05, 2016

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Chapters

  • 00:05Title
  • 00:56Cells and Transfections
  • 03:46Viral Production Assay
  • 05:38Cell Viability Assay
  • 06:28Immune Activation Assay
  • 08:59Results: Evaluation of siRNA Targeting HIV-1 RNA
  • 10:34Conclusion

Summary

Automatic Translation

Methods to evaluate the efficacy and toxicity of RNA molecules targeting post-integration steps of the HIV-1 replication cycle are described. These methods are useful for screening new molecules and optimizing the format of existing ones.

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