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Immunology and Infection

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JoVE Journal Immunology and Infection
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
 

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

Article DOI: 10.3791/54508-v • 07:43 min • September 28th, 2016
September 28th, 2016 •
1, 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health

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Summary
Automatic Translation

Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.

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Keywords: Bacterial Pneumonia Mouse Model Non-invasive Klebsiella Pneumoniae Innate Immune Response Pulmonary Infection Infectious Disease Immunology Biosafety Level 2 Serial Dilutions Optical Density CFU Intranasal Inoculation
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