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1Department of Biochemistry and Molecular & Cellular Biology,Georgetown University Medical Center, 2Department of Nursing,Georgetown University, School of Nursing and Health Studies, 3Department of Human Science,Georgetown University, School of Nursing and Health Studies, 4School of Medicine,Georgetown University Medical Center, 5Department of Pathology and Neuropathology,Medical University of Gdańsk, 6Department of Oncology,Georgetown University Medical Center, 7Department of Pathology,Georgetown University Medical Center
Chapters
- 00:05Title
- 01:13Orthotopic Ewing Sarcoma (ES) Cell Injection and Hypoxia Induction by Femoral Artery Ligation (FAL)
- 04:34Primary Tumor Excision by Leg Amputation
- 06:23Metastasis Monitoring and Primary Tumor Cell Culture
- 08:15Results: Representative Effects of FAL on Primary Tumor Growth
- 10:17Conclusion
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
