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1Centre for Translational Omics, Genetics and Genomic Medicine Deptartment, Great Ormond Street Institute of Child Health,University College London, 2Dementia Research Centre, Institute of Neurology,University College London, 3Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy,University of Gothenburg, 4Neurology Unit, Department of Pathophysiology and Transplantation,University of Milan, 5Great Ormond Street Hospital for Children,University College London
Chapters
- 00:05Title
- 01:05Preparation of Standard Peptides
- 03:33LC-MRM Method Development and Data Analysis
- 05:12Results: High-throughput, Multiplex CSF Assay for Quantification of Neurodegeneration Markers and Detection of ApoE Isoforms
- 06:17Conclusion
We describe a high-throughput, multiplex, and targeted proteomic cerebrospinal fluid (CSF) assay developed with potential for clinical translation. The test can quantitate potential markers and risk factors for neurodegeneration, such as the apolipoprotein E variants (E2, E3 and E4), and measure their allelic expression.
