Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / An Optimized Hemagglutination Inhibition (HI)…
JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine
An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
 

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Article DOI: 10.3791/55833-v • 06:34 min • December 1st, 2017
December 1st, 2017 •
*1, *1, 1, 1, 1, 1,2,3, 1,4
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology, University Hospital Basel
* These authors contributed equally

Chapters

Summary
Automatic Translation

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

Tags

Keywords: Hemagglutination Inhibition Assay Influenza-specific Antibody Titers Vaccine-mediated Immunity Neutralizing Antibodies Mouse Serum Culture Supernatants Antigen Solution RDE Treated Serum Samples Antiserum Serial Dilutions Red Blood Cell Solution Incubation Time Hemagglutination
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter