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Immunology and Infection

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JoVE Journal Immunology and Infection
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
 

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.

Tags

Keywords: Listeria Monocytogenes Experimental Infection Interferon Gamma C57 Black Mice Bacterial Load Survival BHI Agar BHI Broth OD600 PBS Centrifugation Dilution
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