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Flowcytometri-baserede assay for Overvågning af NK cellefunktioner
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Flowcytometri-baserede assay for Overvågning af NK cellefunktioner

DOI:
10.3791/54615-v

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08:17 min

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October 30, 2016

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, , ,
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology,Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy,Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology,Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine,University of Oslo

Chapters

  • 00:05Title
  • 01:01NK Cell Isolation
  • 02:20NK Cell Stimulation
  • 03:52Surface and Intracellular Staining
  • 06:04Results: Representative Healthy Donor NK Cell Stimulation Analysis
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

En enkel og pålidelig fremgangsmåde er beskrevet her for at analysere et sæt af NK cellefunktioner såsom degranulering, cytokin og chemokin produktion inden for forskellige NK-celle delmængder.

Tags

Keywords: Flow CytometryNK Cell FunctionsNatural Killer CellsViral InfectionsMalignant DiseasesPediatricsImmunodeficient PatientsNK Cell IsolationEDTA Peripheral Whole BloodNK Cell StimulationK562 Tumor CellsIL-2IL-15

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