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Fluorescens-aktivert cellesortering for Rensing av Plasmacytoid dendrittiske celler fra mus Bone Marrow
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
DOI:

08:01 min

November 04, 2016

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Chapters

  • 00:05Title
  • 00:49Bone Marrow Cell Isolation
  • 02:30Cell Surface Staining for Fluorescence-Activated Cell Sorting (FACS)
  • 04:09Plasmacytoid Cell FACS Purification
  • 05:48Results: IFNalpha Production by FACS-Sorted pDC
  • 07:10Conclusion

Summary

Automatic Translation

Vi rapporterer en protokoll med fluorescens-aktivert celle sortering å isolere plasmacytoid dendrittiske celler (PDC) med høy renhet fra benmargen av lupus utsatt mus for funksjonelle studier av PDC.

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